FIGURE

Fig. 2.

ID

ZDB-FIG-240305-11

Publication

Iyer et al., 2024 - Chloride transporter ClC-7 is essential for phagocytic clearance by microglia

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Fig. 2.

Late endosomal and lysosomal compartments appear normal in clcn7 mutants. Transgenic expression of (A) mCherry-Rab7 and (B) LAMP1b-mCherry driven under the control of mpeg1 regulatory sequences was similar in clcn7 heterozygotes and mutants. mCherry-Rab7; clcn7^+/−: n=17, mean=26.0, s.d.=12.6, s.e.m.=1.1. mCherry-Rab7; clcn7^−/−: n=8, mean=26.7, s.d.=14.0, s.e.m.=1.3. LAMP1b-mCherry; clcn7^+/−: n=18, mean=22.5, s.d.=12.6, s.e.m.=1.2. LAMP1b-mCherry; clcn7^−/−: n=8, mean=22.5, s.d.=10.3, s.e.m.=1.2. (C) Area and intensity of LysoTracker Red were similar between clcn7 heterozygotes and mutants. clcn7^+/− area: n=14, mean=31.8, s.d.=11.6, s.e.m.=1.3. clcn7^−/− area: n=17, mean=28.8, s.d.=12.8, s.e.m.=1.3. clcn7^+/− intensity: n=14, mean=157, s.d.=41.1, s.e.m.=4.7. clcn7^−/− intensity: n=17, mean=163.3, s.d.=37.5, s.e.m.=3.7. Two-tailed unpaired t-test with Welch's correction was performed to calculate significance; graphs show mean±s.d. Images are representative of two independent experiments. Scale bars: 50 µm. A.U., arbitrary units.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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