FIGURE

Fig. 8

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ZDB-FIG-240229-47
Publication
Altbürger et al., 2023 - The proneural factors Ascl1a and Ascl1b contribute to the terminal differentiation of dopaminergic GABAergic dual transmitter neurons in zebrafish
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Fig. 8

sst1.1+ neurons require Ascl1 function for their proper development. (A-D′) Whole-mount in situ hybridization for sst1.1. Loss of Ascl1 function leads to a severe reduction of sst1.1+ neurons in the prethalamus (A-D′, black arrowheads). A lesser reduction is already detectable in single mutants. ascl1a and double mutants also have less sst1.1+ neurons in the thalamus and the TuD region of the hypothalamus (B,B′,D,D′, non-filled arrowheads). (E–H) Fluorescent sst1.1 in situ hybridization coupled with immunofluorescence for Th. The reduced sst1.1+ neurons (E-H, white arrowheads) do not express Th, but are adjacent to the prethalamic DA neurons, which are also reduced in double mutants. (I–L) Fluorescent in situ hybridization for gad1b/2 and sst1.1. Coexpression analysis with gad1b/2 (I–L) shows that the sst1.1+ neurons in the prethalamus are GABAergic (white arrowheads in L - magnification of the entangled box in K). (A–H) 96 hpf wildtype, single or double ascl1a and ascl1b mutant embryos. (A-D, E-L) Dorsal and (A′-D′) lateral views. (E–H) z-projections of confocal stacks. (I–L) Single confocal planes of a wildtype embryo. Anterior is to the left. For numbers of analyzed embryos see Supplemental Table S2. Abbreviations: Pth, prethalamus; Tel, telencephalon; TuD, tuberal domain, dorsal part. Scale bars: A-D’ - 100 μm, E-K - 50 μm (for scale of L see K).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 505, Altbürger, C., Rath, M., Wehrle, J., Driever, W., The proneural factors Ascl1a and Ascl1b contribute to the terminal differentiation of dopaminergic GABAergic dual transmitter neurons in zebrafish, 587458-74, Copyright (2023) with permission from Elsevier. Full text @ Dev. Biol.