Figure 5

Adult col10a1a^−/− zebrafish have decreased vertebral TMD and altered bone properties. (A) Representative µCT scans (MIPi = Maximum Intensity Projected image) of a year-old adult WT (top) and col10a1a^−/− (bottom) reveal a decreased mineralization and fusion of the caudal fin vertebrae in the mutant. (B) Lateral view of pre-caudal vertebrae 6–8 (L to R) for WT and col10a1a^−/− fish. (C) Representative µCT scan of a vertebra in 3 planar views, showing the two morphometric measurements: vertebral thickness (µm) and vertebral length (µm). (D) Comparison of morphometric measures on precaudal vertebrae 6–8 (n = 4 fish/group) in WT and col10a1a^−/− fish. (E) Line plots generated using the FishCut software version 1.2 revealing significantly decreased TMDs in vertebra (Vert.TMD) and centra (Cent.TMD), as well as neural and centra surface areas (Neur.SA, Cent.SA) in col10a1a^−/− relative to WT, while centra and neural arch volumes (Cent.Vol, Neur.Vol) were not significantly affected (* p < 0.05), (n = 6 fish/group). (F) col10a1a^−/− mutants display fusion of the tail fin vertebra. (n.s. = non significant) significance: *** p < 0.001, and **** p < 0.0001.