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Yap1/Tead activation and inhibition of Stat activity compromises heart regeneration. (A) Concurrent RNAscope in situ hybridization for postnb and immunostaining for MF20 in the 7-dpa control and Epi/FB:caYap1 hearts. White boxes mark the injury area, under which are their respective zoom-in images. White dashed lines indicate approximate injury border. Scale bar, 50 μm. (B) Quantification of the area of postnb expression in the wound area on sections comparing Epi/FB:caYap1 and control fish. n = 5 hearts. (C) qRT-PCR analysis of col1a1b, postnb, and fn1a in Epi/FB:caYap1 hearts and control hearts at 7 dpa, respectively. (D) AFOG staining for controls, Epi/FB:caYap1, Epi/FB:dnYap1, and EC:dnStat fish hearts at 30 dpa, respectively. Black boxes mark the injury area. Scale bar, 100 μm. (E) Quantification of scar area at 30 dpa. n = 5 hearts. (F) Concurrent RNAscope in situ hybridization for fosl1a and immunostaining for MF20 in the 7-dpa control and EC:dnStat hearts. White dashed lines indicate the approximate resection plane. Scale bar, 50 μm. (G) Quantification of the fosl1a-positive area in the white boxed area comparing control and EC:dnStat fish. n = 5 hearts. (H) qRT-PCR analysis of fosl1a, tal1, and raldh2 in EC:dnStat transgenic fish and controls at 7 dpa, respectively. (I) Images of sections of 7-dpa hearts from transgenic fish carrying cLEN fragments. Scale bar, 50 μm. P value calculated with two-tailed Student’s t test. *P < 0.05. **P < 0.01.
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