Fig. 2
Establishment of TAA-induced liver fibrosis model in zebrafish. (A) The Sirius red staining of zebrafish. Figures are magnified at ×100 (n = 8). (B) H&E staining of zebrafish. Figures are magnified at ×100 (n = 8). (C?G) The qPCR analysis of PPAR?, FASN, Col1?1b, ?-SMA, and TNF-? mRNA expression in zebrafish larvae (n = 3 or 4). The control group consisted of untreated zebrafish at 8 dpf. The mRNA expression was normalized to ?-actin mRNA expression and presented as a fold change compared with the control group. ns denotes no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. |