Fig. 1

Folliculogenesis in zebrafish and changes in protein profile. (A) Stages of zebrafish follicles. PG (<150 μm); PV (~250 μm); EV (~350 μm); MV (~450 μm); LV (~550 μm); FG (>650 μm). (B) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles during folliculogenesis. (C and D) 2D protein profiles of PG and PV stages. Total proteins of PG and PV follicles (80 μg) were used for IEF with IPG strips (7 cm, pH 3–10 linear) respectively. 2D gels were stained with Coomassie blue. The differentially expressed proteins were identified and marked by numbers, and the list of identified proteins is shown in Supplementary Table S2. Some proteins characterized in both PG and PV samples did not exhibit any evident alterations (e.g. #22, 23, 24, 33, 34, and 38) between the two stages, and these proteins can serve as internal controls for loading. (E) Selected proteins down-regulated in PV follicles. (F) Selected proteins up-regulated in PV follicles. Eif4enif1, eukaryotic translation initiation factor 4E nuclear import factor 1 (gi|61,806,649); Cpeb1b, cytoplasmic polyadenylation element-binding protein 1b (gi|82,249,026); Ybx1, Y box-binding protein 1; Lsm14, LSM14 homolog B-like (gi|239,050,742); Pabpn1l, poly(A) binding protein nuclear 1 like (gi|189,521,749); Igf2bp3, insulin-like growth factor 2 mRNA binding protein 3 (gi|18,858,571); Cirbpa, cold inducible RNA binding protein a (gi|62,955,567); Elavl2, ELAV Like RNA Binding Protein 2 (novel protein containing RNA recognition motifs) (gi|94,732,878); Ddx4, DEAD-Box helicase 4 (gi|2,558,535); P4hb, prolyl 4-hydroxylase beta subunit (gi|193,788,703); Pdia3, protein disulfide isomerase A3 (gi|27,881,963); Eno3, enolase 3 (gi|47,551,317); Pgk1, phosphoglycerate kinase 1 (gi|41,388,972); Ldhba, lactate dehydrogenase Ba (gi|28,277,619); Hsp90b1, heat shock protein 90 beta 1 (gi|38,016,165). The two images shown for Ybx1 represent the protein located at the acidic and basic sides of the gel, respectively.