Fig. 2

A primary drug screen of the LOPAC1280 library reveals 48 hit compounds that consistently down-regulate otic vcanb expression in adgrg6tb233c−/− embryos. (A) Overview of vcanb staining scores in the primary screening assay. Green, hits (score <6); grey, non-hits (score 6–9); red, toxic/corrosive compounds. (B) Total vcanb staining scores (summed from 3 embryos) from all compounds in the primary screen, including 64 initial hits (green). Library compounds are ordered along the x axis based on structural similarity. Red data points, toxic/corrosive compounds; grey, no effect on vcanb expression (non-hits); green, reduced vcanb expression (initial hits); blue, additional compounds selected for retest based on structural similarity with hits. (C) Comparison of lipophilicity of initial hit (green) and non-hit (grey) primary screen compounds. ****p < 0.0001, Welch's two-tailed t test. (D) Retesting 82 compounds revealed 48 (green and blue) that consistently down-regulated vcanb expression after two retests.