Functional connectivity between transcriptomically defined V2a IN and MN subtypes. a, Monosynaptic connections between presynaptic Esrrga+ (top), Shox2+ (middle) or vAChTa+ (bottom) V2a INs and postsynaptic slow/Esrrga+, intermediate or fast MNs, respectively. b, Morphological reconstruction of the connected V2a IN–MN pairs (black, soma and dendrites; red, V2a IN axons). c, EPSP amplitude of V2a IN–MN connected pairs of each module (n = 9, top; n = 11, middle; n = 9, bottom). d, Two-photon ablation of slow/Esrrga+ but not of fast/vAChTa+ V2a INs affected slow explorative swimming. Graphs show the cumulative distribution or average swimming velocity in control (Ctrl) and zebrafish with ablated (Ablt) slow/Esrrga+ (magenta, left) or fast/vAChTa+ (blue, right) V2a INs (mean ± s.e.m.; two-tailed Student’s t-test, *P = 0.0111; Esrrga+ V2a IN ablation, n = 5 controls and n = 6 ablated; vAChTa+ V2a IN ablation, n = 6 controls and n = 6 ablated; bl, body length). e, Two-photon ablation of fast/vAChTa+ (blue, right), but not slow/Esrrga+ (magenta, left) V2a INs affected touch-induced fast swimming (mean ± s.e.m.; two-tailed Student’s t-test, *P = 0.0102; Esrrga+ V2a IN ablation, n = 5 controls and n = 6 ablated; vAChTa+ V2a IN ablation, n = 6 controls and n = 6 ablated). f, Sound-induced escape behavior was not affected by ablation of slow/Esrrga+ (magenta, left) or fast/vAChTa+ (blue, right) V2a INs (mean ± s.e.m.; two-tailed Student’s t-test, P > 0.05; Esrrga+ V2a IN ablation, n = 5 controls and n = 6 ablated; vAChTa+ V2a IN ablation, n = 6 controls and n = 6 ablated). In c–f, boxes are bound by the 25th and 75th percentiles, whiskers extend from minimum to maximum. Source data
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