Fig. 3

Zebrafish fbxo3 enhances virus replication in SVCV-infected EPC cells. (A) Overexpression of fbxo3 reduced cell survival of EPC cells after SVCV infection. EPC cells were transfected with 2 μg of Myc-fbxo3 or empty vector. At 24 h posttransfection, the cells were infected with SVCV at the dose indicated for 48 h. Then, the cells were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (B) Overexpression of fbxo3 increased virus titers in EPC cells after SVCV infection. The culture supernatant was collected from EPC cells infected with SVCV (MOI of 100), and the viral titer was measured by plaque assay. The results are representative of three independent experiments. (C–E) Overexpression of fbxo3 increased copy number of SVCV-related genes in SVCV-infected EPC cells. EPC cells were transfected with Myc-fbxo3 or empty vector and infected with SVCV (MOI of 10). After 24 h, total RNAs were extracted for examining the mRNA levels of the G protein (C), N protein (D), and P protein (E) of SVCV by qRT-PCR analysis. Data are presented as means ± SEM of three biological replicates. **p < 0.01, ***p < 0.001, ****p < 0.0001.