Fig. 2

Effect of MATAL1 knockdown on cell proliferation and metastasis in vivo. HeLa and CaSki cells were transfected with NC and siRNA against MATAL1, respectively, and labeled cells were then transplanted into larval zebrafish to evaluate cell proliferation and metastasis in vivo, as described in the Materials and methods section. (a and b) Representative photos of zebrafish injected with HeLa cells were taken using a confocal laser scanning microscope for each treatment. To quantify the proliferation and metastasis of injected cells, the zebrafish larvae were imaged with stereomicroscope. The fluorescence of HeLa cells that (c) resided in the fish yolk or (d) trunk was quantified using Image J software, and the values were normalized against those of the NC (n = 10). (e-h) Representative pictures (left panel) and quantified results (right panel) are shown for CaSki cells, as above (n = 10) (scale bar: 100 μm). NC, negative control.