FIGURE

Fig. 2

ID

ZDB-FIG-231218-7

Publication

Wang et al., 2023 - Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Functional gametes were obtained using iPGCT.

a Schematic diagram for iPGCT using Tg(cmv:GFP) embryos as iPGCT donors and Tg(cmv:mCherry) embryos as iPGCT hosts. b After iPGC transplantation, host embryos expressed mCherry and germ cells expressed GFP. c Immunofluorescence detection of Vasa in GFP-positive germ cells of iPGCT gonads at 32 dpf. Note that gonadal somatic cells expressed mCherry. d H&E staining of Tg(cmv:GFP) and iPGCT testes. SC spermatocyte, ST spermatid, SZ spermatozoa. e Immunofluorescence detection of Sycp3 and PCNA showing normal meiosis and mitosis of germ cells in iPGCT testis and control testis at 90 dpf. f Immunofluorescence detection of Vasa in GFP-positive germ cells of iPGCT testes at 90 dpf. Rectangular boxes 1 and 2 show the development of gonads on both sides of the iPGCT embryo, respectively. g The iPGCT fish expressing mCherry produced sperm expressing GFP, and F1 and F2 generations expressing GFP were obtained. A representative example of three replicate is shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.