FIGURE 2

Effect of Aquilaria sinensis leaf tea on the sleep of zebrafish with sleep disturbance. (A) The schematic diagram shows sleep disturbance treatment. Sleep disturbance was performed for three consecutive days between 23:00 and 03:00 (ZT14-ZT18) from 5 dpf to 8 dpf. (B) Zebrafish larvae (5 dpf) were extract treated with six concentrations of A. sinensis leaf tea (0.00, 0.50, 0.75, 1.00, 1.25, and 1.50 g/L; n = 48) and placed in a behavior-tracking system. (C,D) Sleep and waking activity traces (±SEM) of zebrafish larvae treated with (A)sinensis leaf tea. The gray box indicates sleep disturbance time. (300 lux light on 23:00–03:00) (n = 48). (E) Quantification of total sleep across day time (9:00–23:00), sleep disturbance time (23:00–03:00), and night time (03:00–09:00) on 6 dpf (n = 48). (F) Sleep bout number between 23:00–03:00 (n = 48). (one-way ANOVA, Tukey’s post hoc test, Different lowercase letters indicate significant differences between various concentrations).