Fig. 8

Dendritic profiles of tectal cells with CD synaptic inhibition.

a Dendritic profile of a recorded GFP-positive neuron labeled with sulforhodamine (red) in the Tg(pou4f1-hsp70l:GFP) line (cyan). Scale bar 20 μm. Profiles of fluorescence intensity (top inset, peak-scaled) were measured separately in the red and cyan channel of the dual color image (center) across the PVL and neuropil (rectangular area in center image). The recorded neuron received CD inhibitory postsynaptic currents associated with swimming (bottom inset). Scale bars MNR: 50 μV, I_post 50 pA, time: 500 ms). b Average dendritic profiles of recorded GFP-positive neurons, for cells with motor-related, CD inhibition (top) and without CD inhibition (bottom). Profiles were aligned with respect to the PVL/neuropil boundary (vertical dashed line) and the peak of the SFGS and averaged (cyan traces, mean ± SEM). Profiles of neurons (red) exhibit dendrites mostly in SAC, SGC and central SFGS, but not in more superficial layers. Shaded areas indicate approximate extent of neuropil layers. c Same as in a, but for a GFP-negative neuron patched in the Tg(pou4f1-hsp70l:GFP) line. Note the bistratified dendritic profile, with the upper dendrites in target layers more dorsal to those of GFP-positive neurons. d Same as in b, but for GFP-negative neurons patched in the Tg(pou4f1-hsp70l:GFP) line. Note the peak of dendritic profiles superficial to the SFGS (arrow). Source data are provided as a Source Data file.