FIGURE

Figure 3

ID

ZDB-FIG-231127-27

Publication

Hall et al., 2023 - Reduction in N-Acetylglucosaminyltransferase-I Activity Decreases Survivability and Delays Development of Zebrafish

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Figure 3

Confirmation of decreased Mgat1b expression, and an increase in oligomannose N-glycans in mutant strain of adult and larvae fish. Lectin blots of whole cell lysates of adult brain (A), and larvae at 24 dpf and 6 dpf from Wt AB and Mgat1b^−/− mutant zebrafish (B). Separated proteins were probed with Phaseolus vulgaris Erthroagglutinin (E-PHA) or Galanthus nivalis lectin (GNL). Coomassie blue (CB)-stained gel demonstrating equal protein loads among the samples. Lines adjacent to the blots denote protein markers (in KDa) 250, 150, 100, 75, 50, 37. Bar graphs represent Mgat1b mRNA levels from Wt AB and Mgat1b^−/− mutant zebrafish relative to housekeeping genes β-actin (C) and ELFα (Figure S1). Data are presented as mean ± SEM, n ≥ 3 and were all compared using Student’s t-test (** p < 0.005, * p < 0.02).

Expression Data

Gene:	mgat1b
Fish:	AB mgat1b^{ecu300/ecu300} (AB)
Anatomical Terms:	brain whole organism
Stage Range:	75%-epiboly to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mgat1b^{ecu300/ecu300} (AB)
Observed In:	brain brain alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity whole organism whole organism alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
Stage Range:	75%-epiboly to Adult

Phenotype Detail

Acknowledgments

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