FIGURE

Figure 4

ID

ZDB-FIG-231116-131

Publication

Finotto et al., 2023 - Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma

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Figure 4

Macrophages shift toward an immunosuppressive phenotype upon co‐culture with patient‐derived GSCCs

PCA plot of macrophage population without TransMos shows two distinct macrophage subclusters (MC1‐2). PCs were calculated using the 2,000 most variable genes. PC1 and PC2 are shown on the PCA plot.

Macrophage subcluster distribution for the different samples.

Representation of original samples on the PCA plot.

Pseudotime analysis along PC1 axis. Violin plots depicting the PC1 values of each single cell, split up by sample.

Heatmap of the 100 most significant temporally expressed genes along the PC1 axis, constructed by fitting a generalized additive model with the PC1 value as a LOESS term (see Materials and Methods).

Dot plot of cell–cell communication analysis using CellPhoneDB. Depicted are L:R pairs for GSCC ‐ macrophage signaling across all GSCCs, ranked by mean log₂ expression. Each dot size shows the log₂ mean of expression values and dot color indicates the P‐value for the listed L:R pairs (x‐axis) in the respective GSCCs (y‐axis). Only top 50 significant L:R pairs, with cut‐offs of P‐value ≦ 0.05 are shown. The P‐values were generated by CellPhoneDB, which uses a one‐sided permutation test to compute significant interactions.

Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.