Fig. 5

Commonly deregulated genes in HRAS-OE hearts and regenerating hearts. (A,B) Experimental design for transcriptomic analysis of oncogenic HRAS-expressing hearts (A) and regenerating hearts (B) with the controls. The transgenic strains are indicated above the heart drawing. Three hearts were pooled per samples and biological triplicates were used for each experimental condition. The HRAS-OE heart transcriptome shows similarity with 7 dpci regenerating heart, compared with their controls. (C) Heatmap with hierarchical clustering of log2-transformed normalized read counts for 2250 common differentially expressed (DE) genes in cmlc2/GFP-HRAS and in 7 dpci compared with their relevant control (padj<0.05, absolute fold change >1.5). Columns indicate the expression average of the three replicates for each group. Rows indicate individual genes. Red indicates high expression and blue indicates low expression. (D) Selected enriched gene ontology terms for the 1618 common upregulated genes in HRAS-OE and regenerating hearts representatively shown in C. (E) Selected enriched gene ontology terms for the 632 common downregulated genes in HRAS-OE and regenerating hearts representatively shown in C. (F-I) Immunofluorescence analysis of identified candidate genes commonly deregulated in HRAS-OE and regenerating heart at 7 dpci. (F) Extracellular matrix protein Collagen XII is enriched in the ventricle HRAS-expressing myocardium and the peri-injury zone at 7 dpci. (I) The sarcomeric protein Myomesin is downregulated in the ventricle HRAS-expressing myocardium and the peri-injury zone at 7 dpci. n=5.