Fig. 1

Zebrafish larvae toxicity test showed that ronidazole and furazolidone had the same low toxic side effects as Mtz. (A) Experimental schematic illustrating the stages of five Mtz analogs treatment and analysis at BT, Reg 0 h, Reg 24 h, and Reg 48 h. (B) Cumulative survival of different larvae after been treated with Mtz analogs at 10 mM; each group n = 30 larvae. Data were analyzed by the log-rank Mantel–Cox test, *p < 0.05; **p < 0.01; ***p < 0.001. All samples were run in triplicate. (C) BF images showing the body phenotype treatment with Mtz analogs at 6 dpf. (D) Quantification of the phenotypic proportion of different Mtz analogs from 2 to 10 mM after 24 h treatment; n = 30 larvae. BF, bright field; BT, before drug treatment; dpf, days postfertilization; Mtz, metronidazole; NS, no significant difference.