Fig. 3

Fsta restricts the transmission of spaw in the LPM and prevents early activation of lefty1 in the forebrain of zebrafish. (A) PA propagation of spaw from 12 to 17-somite stage (ss) in WT and fsta?5/?5 embryos. myod1 is used to indicate the development stage. Red triangles indicate the anterior boundary of spaw expression in the left LPM. Blue triangles indicate the anterior boundary of spaw expression in the right LPM. (B) The violin plot shows the statistics of (A), rectangles indicate the middle two quadrants in the distribution and black dots indicate the median. Negative numbers indicate that the anterior boundary of spaw has crossed the first somite. The ratio and numbers of embryos that have spaw expression in the left LPM of WT and fsta?5/?5 at 12ss are shown in the histogram. (C) Forebrain expression of lefty1 in WT and fsta?5/?5 embryos at the indicated stages. Statistics are shown on the right, ?n? indicates the numbers of examined embryos. At least 20 embryos were examined in each experiment. (Scale bar, 200 ?m.)