Fig. 2

Regeneration of the perineurial structure ensheathing the motor nerves following recovery from acute hyperglycemia. (A) A schematic outlining the treatment and recovery schedule. (B) Following the schedule of treatment described in (A), there was no significant (ns) difference in whole-body glucose levels of Tx22 fish compared to C22 fish, P = .1284. (C and D) Lateral view of C22 and Tx22 Tg(nkx2.2a:mEGFP);Tg(NBT:DsRed) zebrafish. Following the recovery period, perineurial glia (green) were associated with peripheral motor axons (red) in both C22 (C, asterisk) and Tx22 (D, asterisk) fish. (E) The percentage of perineurial-ensheathed motor axons did not differ between Tx22 and C22 fish following recovery, P = .4730. (F) Similarly, no difference in the length of perineurial-ensheathment was observed between C22 and Tx22 fish following the recovery period, P = .6890. (G and H) Representative images of C22 and Tx22 Tg(nkx2.2a:mEGFP) zebrafish antibody labeled against ZO-1 (red). ZO-1+ tight junction proteins were continuous along the length of the perineurium in C22 fish (G″, arrow). ZO-1+ tight junction proteins also appeared to be continuous in Tx22 fish (H″, arrow). (I) ZO-1+ fluorescent intensity (standardized to nkx2.2a) was not significantly different between Tx22 and C22 fish, P = .8400 (au, arbitrary units). All values are means ± SEM. Scale bars = 50 μm.