FIGURE

Fig. 1.

ID
ZDB-FIG-231012-10
Publication
Harish et al., 2023 - Real-time monitoring of endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation
Other Figures
(all 7)
All Figure Page
Back to All Figure Page
Fig. 1.

Generation and characterization of the Tg(fgf8a:fgf8a-EGFP) fish line. (A) Knock-in strategy: EGFP was inserted between exons 2 and 3 of the endogenous Fgf8a locus using CRISPR/Cas9. Exon sequences are shown as numbered blocks, separated by introns. The open reading frame sequence is in blue. Red arrowheads indicate the sgRNA target sites (ts). (B) In situ hybridization against fgf8a and gfp in 16-somite stage (ss) embryos. Scale bars: 100 μm. (C) Fgf8a-EGFP fluorescence in the dorsal embryonic margin (arrow) at late gastrula, PSM at 20 ss, as well as at the basal (arrowheads) and lateral (arrows) sides of the MHB neuroepithelia 24 h post fertilization (hpf). Orientations of embryos are shown schematically in insets. Red rectangles outline the locations imaged. sh, shield. Scale bars: 50 μm. (D) Comparison of homozygous fgf8a-EGFP with wild type and homozygous ace mutants at 24 hpf. Homozygous viability and normal structure of the MHB (arrowhead) in the fgf8a-EGFP transgenics confirms the functionality of the fgf8a-EGFP knock-in line. Lateral views are shown. Scale bars: 100 μm.
Expression Data
Gene:
Fish:
Anatomical Terms:
Stage Range: 90%-epiboly to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.