opn8 is expressed in Pax6+ cells in the INL and GCL. (A) Fluorescent ISH against opn8 (red) followed by immunohistochemistry against an amacrine and RGC marker (Pax6; green) on stage 41 Xenopus laevis transverse retinal section. DAPI (blue) stained the nucleus. ONL, INL, and GCL indicated with bars. White arrows point to opn8-expressing cells that are Pax6+ INL cells, while yellow arrows indicate Pax6+ GCL cells. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium. Scale bar = 50 μm. (B) Distribution of opn8+/Pax6+ cells between the INL and the GCL of stage 41 retinas. Each dot represents the percentage of cells in one central retina section (n = 8 sections); Lines are the mean with 95% confidence interval.
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