FIGURE

Fig. 5

ID

ZDB-FIG-230915-32

Publication

Vaparanta et al., 2023 - STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth

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Fig. 5

Figure 5. Stat5b is necessary for cardiac growth and function in zebrafish embryos

A, B. Confocal images (A) and quantification (B) of immunofluorescence staining of myosin heavy chain and Stat5b in hearts of 4 dpf zebrafish embryos injected with CRISPR/Cas9 and either control or stat5b-targeting gRNA at one-cell stage. The myosin immunoreactivity was used to quantify the average thickness of the ventricular wall and the cross-sectional area of the ventricles. Stat5b staining intensity was also quantified. One dot in the boxplots corresponds to one heart (control gRNA: n = 14, stat5b gRNA n = 13; biological replicates from one of three replicate experiments). Unpaired two-tailed T-test was used for statistics. Scale bar 50 μm.
C, D. Still frame phase contrast images of in vivo imaging of the zebrafish embryo hearts (C) and quantification of the ejection fraction (D). The 4 dpf embryos were treated as in A. Ejection fraction was quantified from the videos. One dot in the boxplots corresponds to ejection fraction of one heart (for each group n = 22; biological replicates from one of three replicate experiments). Unpaired two-tailed T-test was used for statistics. Scale bar 50 μm.

Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values.

Source data are available online for this figure.

Source Data for Figure 5 [embr202256689-sup-0019-SDataFig5.zip]

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Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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