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l-CaD colocalizes with actin in filopodia, and the knockdown of l-CaD downregulates N-cadherin A Representative fluorescent merge images of PC3 cells transfected with siNeg1 or siCaD-1 and stained with antibodies recognizing actin and l-CaD. The lower row shows separate images of actin, l-CaD, and merge in a close-up of the upper siNeg1 image. B Representative fluorescent images of PC3 spheroids transfected with siNeg1, siNeg2, or siCaD-1, stained with N-cadherin, and counterstained with DAPI. C Barplots depicting N-cadherin protein intensity in spheroids formed by transfected PC3 cells shown in B. Average was calculated from multiple individual spheroids (N = 22) measured for mean intensity. The mean and SD are shown (ns = not significant, *p < 0.05, and **p < 0.01 as determined by t-test). D Representative fluorescent images of DU145 spheroids transfected with siNeg1, siNeg2, siCaD-1, or siCaD-2 stained against N-cadherin. E Barplots depicting N-cadherin protein intensity in spheroids formed by transfected DU145 cells shown in D. Average was calculated from multiple individual spheroids (N = 30) measured for mean intensity. The mean and SD shown (ns = not significant, ***p < 0.001, and ****p < 0.0001 as determined by t-test). F Representative fluorescent images of DU145 spheroids transfected with siNeg1, siNeg2, siCaD-1, or siCaD-2 stained against ZEB1. G Barplots depicting ZEB1 protein intensity in spheroids formed by transfected DU145 cells shown in F. Average was calculated from multiple individual spheroids (N = 58) measured for mean intensity. The mean and SD shown (ns = not significant, **p < 0.001, ***p < 0.001, and ****p < 0.0001 as determined by t-test).
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