Figure 2

Establishment of cdh23 gene knockout lines. (A) The schematic diagram of the cdh23 gene target site. The gene is 450.8 kb in length and has 72 exons. Exon 18 is the target for CRISPR/Cas9 gene editing in zebrafish cdh23. (B) Design of cdh23 gene knockout target, the uppercase letter is the exon, the lowercase letter is the intron, “___” is the detection primer, “ ~ ~ ~ ~” is the target site sequence, and “=====” is the PAM sequence. The distance of the two target sites is 115 bp; (C,D) Partial DNA and the corresponding amino acid sequence of cdh23 line 1 and line 2 mutant, respectively. The blue box indicates the missing sequence. Line 1 has a total deletion of 34 bp, and protein translation is terminated prematurely. Line 2 missed 143 bp, and protein translation also terminated prematurely. (E,F) DNA agarose gel of cdh23 line 1 and line 2, respectively.