FIGURE 11

Expression of vax1 and vax2 in larval zebrafish. (A–G)vax1. (H–N)nrip1a. (A–D,H–K) Projections of representative whole, imaged eyes. (E–F”,L–M”) Single z slices obtained from the same preparations. Note reduced expression domain of lws2 (cyan), and expanded expression domains of lws1 (magenta) in T3-treated (B,D,I,K) vs. controls (A,C,H,J); the vax1 (yellow, A–D) and vax2 (yellow, H–K) expression domain did not appear to change. (E’–F”) Enlarged images of regions within box of panels (E,F, respectively). (E’,F’) All imaging channels merged. (E”,F”)vax1(L’–M”) Enlarged images of regions within box of panels (L,M, respectively). (L’,M’) All imaging channels merged. (L”,M”)vax2(G) qPCR quantification of vax1 transcript abundance in pooled samples of whole larvae, n = 4 biological replicates per condition, p = 0.123. (N) qPCR quantification of vax2 transcript abundance in pooled samples of whole larvae, n = 5 biological replicates per condition, p = 0.312. n.s., not significant.