Fig. 2

A) qRT-PCR analysis of CD163, CD206, CCL1, CCL22, IL-10, ARG-1, IL-12, CD86, iNOS and HLA levels in M-DM after 24 h exposure to conditioned medium (CM) from control (Mneo) or Bcl-xL overexpressing (Mxl90, Mxl7) melanoma cells. The results are reported as percentage of mRNA variation in macrophages exposed to CM derived from Bcl-xL overexpressing cells versus control ones. B) TGF-β protein secretion quantified by ELISA essay in M-DM stimulated as reported in A). The results are reported as fold of TGF-β release in M-DM exposed to CM from Bcl-xL overexpressing cells versus control ones. A,B) p-values were calculated between M-DM exposed to CM from Bcl-xL overexpressing cells and those exposed to CM from control cells. C) Representative images (left panels) and relative quantification (right panel) of M-DM migration in response to CM from Mneo (CM Mneo), Mxl90 (CM Mxl90) or Mxl7 (CM Mxl7) cells. The quantification was performed by counting the number of migrated cells in at least 10 fields for each condition. The results are reported as percentage of migrated cells/field. p-values were calculated between the percentage of M-DM migrated in response to CM derived from Bcl-xL overexpressing cells, respect to the CM from control melanoma cells. A) The mean ± SEM and B,C) mean ± SD of three independent experiments is reported, *p < 0,05, **p < 0.01