Fig. 6

Figure 6. GenX suppressed the respiratory burst ex vivo at 24 hr. After isolation from individual human donors, neutrophils were dosed with vehicle control, (A) PFHxA, (B) GenX, or (C) PFBS and distributed into a 96 well plate. At 24 hr, cells were stimulated with PMA to produce ROS, which was detected with DHR. Maximum fluorescence values are reported here. The entire fluorescence (AUC) values are provided in Supplemental Figure S18. Fluorescence of DHR was measured for 2.5 hr on a fluorescent plate reader set to 37°C. Wells with no cells but with PMA and DHR, and cells receiving no PMA were included as controls. Cells treated with Bis I, a protein kinase C inhibitor, were included as a positive control for inhibition of the respiratory burst. Data shown are from six human donors (3 male, 3 female). Each biological replicate included 3-6 technical replicates/treatment group. Individual symbols represent individual wells from a 96-well plate. Statistical significance (*p < 0.05) was determined by a two-way ANOVA with a Dunnett’s or Sidak’s post-hoc test for pair-wise comparisons to the vehicle control.