Figure 5.

Washout kinetics of the pharmacological treatments in the larval and embryonic zebrafish brain. A, Boxplots showing the overall mean distance per larva from 5-dpf larvae, following acute application of veratridine (green), in control conditions (blue), following acute application of TCNF (red) and after 24–48 h of wash for each treatment (light boxplots). For all boxplots the p of normality test for at least one condition was >0.05, therefore KW test was used, 28 < n < 117 fish from three independent experiments. B, C, Boxplots showing parameters of Ca²⁺ transients in the telencephalon of 2-dpf embryos, following acute veratridine application (green), in control conditions (blue), following acute TCNF application (red) and following 6–10 h of wash for each treatment (light green and light red boxplots). B, Incidence of Ca²⁺ transients. BFW ANOVA test. C, Frequency of Ca²⁺ transients, KW test. 258 < n < 318 values from three independent experiments. *p < 0.05, **p < 0.01, ****p < 0.001, ns = non significant.