Figure 1

Telomerase inhibition altered RELA proto-oncogene NF-kB subunit (p65) nuclear levels. (A,B) Cells were treated with indicated concentrations of BIBR1532 (BIBR) or dimethyl sulfoxide (DMSO) as control, for 24 h. Levels of relative mRNA expression for p65 in 4134/Late (A) and BL41 (B) cells are shown. Data represent the mean and standard deviation (SD, bar) from three separate experiments. (C,D) Cells treated with BIBR at indicated concentrations, or DMSO as control, for 24 h were processed to obtain cytoplasmic and nuclear extracts. Representative Western blots showing cytoplasmic and nuclear protein levels of p65, phospho-p65 (p-p65), telomeric repeat binding factor 2 (TRF2), and α-tubulin in 4134/Late (C) and BL41 (D) cells are shown. α-tubulin and TRF2 were used as loading controls for the cytoplasmic and nuclear fractions, respectively. The original Western blots are shown in File S1. Graphs next to the blots show the values in arbitrary units of densitometric analysis performed with ImageJ software. Data represent the mean and SD (bar) from three separate experiments. A significant difference between values in BIBR-treated vs. DMSO-treated cells is shown: * p < 0.05; ** p < 0.01; ns: not significant.