Hyperinflation of the swim bladder in zebrafish lateral line mutants (lhfpl5b−/−). (A) Representative examples of over-, regular- and under-inflated swim bladders (arrows) in larval zebrafish at 6 dpf (top: lhfpl5b−/−; middle and bottom: wild-type). (B) Correlation between buoyancy and swim bladder volume. Line of best fit applied through the combination of mutant and wild-type data (linear model, R2=0.8574). (C) Percentage of larvae that exhibit over-, regular and under-inflation of the swim bladder, as defined by positive, neutral and negative buoyancy. Legend in D applies to both C and D. A χ2 test was used to determine significance. (D) Swim bladder volume (mm3) in wild-type and lhfpl5b mutant larvae at 6 dpf. A Welch's t-test was used to determine significance. ***P<0.001, ****P<0.0001. Scale bars: 0.2 mm.
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