Figure 1

Schematic illustration of the experimental strategy and summary of RNA-Seq results.

(A) CD34⁺ cells were isolated from blood (PBMCs) obtained from CMS or non-CMS subjects, pooled, and treated with hypoxia or room air (as control). Following the treatment, total RNA was isolated, and the quality was determined with TapeStation. Ribosome-depleted (ribodepleted) libraries were generated and sequenced. The candidate hypoxia-responding lncRNAs were identified and prioritized for further qRT-PCR–based evaluation and functional analyses. (B) Hypoxia treatment induced distinct transcriptional responses in CMS and non-CMS cells. A total of 426 or 1,702 hypoxia-induced DEGs were identified in the CMS and non-CMS cells, respectively, with little overlap. Further annotation revealed a distinct group of 5 lncRNAs in the CMS DEGs and 36 lncRNAs in the non-CMS DEGs, suggesting specific lncRNA-mediated hypoxia responses between CMS and non-CMS subjects (see also Supplemental Table 1).