FIGURE

Fig. 4.

ID

ZDB-FIG-230518-105

Publication

Keatinge et al., 2023 - Unexpected phenotypic and molecular changes of combined glucocerebrosidase and acid sphingomyelinase deficiency

Other Figures

(all 6)

All Figure Page

Back to All Figure Page

Fig. 4.

Gaucher cell accumulation in the retina of gba1^−/−;smpd1^−/− double mutants. (A) Analysis of the numbers of 4C4-positive cells within the retina revealed comparatively significant increases of ∼50% in both gba1^−/− (P=0.0026) and gba1^−/−;smpd1^−/− mutants (P=0.0193) compared to those in WT, despite enhancement of key glycolipid levels in the double mutants. 4C4-positive cell counts in smpd1^−/− were not statistically different to those in WT. Data represented are the mean±s.d. *P<0.05; **P<0.01 (two-way ANOVA). n= 4 for all groups. (B) 4C4-positive cells in the retina of the gba1^−/− and gba1^−/−;smpd1^−/− double mutants were localised throughout the retina and appeared larger and rounder (arrows) compared to ramified WT 4C4-positive cells (arrowheads). Scale bar: 50 μm.

Expression Data

Antibody:	Ab-4C4
Fish:	WT gba1^sh391/sh391 smpd1^sh696/sh696 gba1^sh391/sh391; smpd1^sh696/sh696
Anatomical Term:	retina microglial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gba1^sh391/sh391 smpd1^sh696/sh696 gba1^sh391/sh391; smpd1^sh696/sh696
Observed In:	retina microglial cell
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.