Fig. 2.

Aging reduces the regeneration of turquoise killifish caudal fin. Old and young turquoise killifish of both sexes were subjected to caudal fin regeneration assay. In total four groups were used (young male, old male, young female, old female). Young were 5 weeks of age and old 12 weeks when entering the experiment. (A) Microscopy images of turquoise killifish caudal fins before, immediately after and 8 days after resection. Scale bar: 1 mm. (B) Kaplan–Meier survival curve of turquoise killifish strain MSCZ222 housed in Turku, Finland. n=77. (C) Quantification of the total regeneration of the caudal fin. Two-way ANOVA with age and time as factors was used for statistical analysis, n=8 per group. All time points were used in the statistical analysis. (D) Thickness of regenerating zone. Two-way ANOVA with age and time as factors was used for statistical analysis. All timepoints were used in the statistical analysis. (E) Comparison of the total regeneration in male and female turquoise killifish caudal fin. In E, two-way ANOVA with sex and age as factors was used for statistical analysis. n=8 per group.