FIGURE

Fig. 2.

ID

ZDB-FIG-230420-79

Publication

Snodgrass et al., 2023 - Therapeutic targeting of vascular malformation in a zebrafish model of hereditary haemorrhagic telangiectasia

Other Figures

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Fig. 2.

eng^mu130 mutant embryos have increased basilar artery diameter and increased number of endothelial kugeln. (A) Representative maximum-intensity projection of Tg(kdrl:Hsa.HRAS-mCherry)^s916 zebrafish brain vasculature at 72 hpf. Black arrowheads indicate individual kugeln. Scale bars: 150 μm/50 μm (inset). Three-dimensional reconstruction of the same image shown in green is on the right. (B) Representative maximum-intensity projection of the basilar artery (BA) at 72 hpf. Black arrowheads indicate individual kugeln. White bars indicate where diameter of the BA was measured. Scale bar: 50 μm. Three-dimensional reconstructions of the same images shown in green are on the right. (C) BA diameter in eng^mu130 mutants and WT siblings (unpaired two-tailed Student's t-test, 10-12 animals/group). (D) eng mutation did not alter endothelial cell (EC) number in the BA (unpaired two-tailed Student's t-test, 10-12 animals/group). (E) Kugel number per animal was increased in eng^mu130 mutants at 52 and 74 h post fertilisation (hpf) but not at 100 hpf. *P<0.05; ns, not significant (Mann–Whitney U-test, 8-27 animals/group).

Expression Data

Gene:	mCherry
Fish:	s916Tg eng^mu130/mu130; s916Tg
Anatomical Term:	brain vasculature plasma membrane
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eng^mu130/mu130; s916Tg
Observed In:	basilar artery blood vessel endothelial cell blood vessel endothelial cell plasma membrane bounded cell projection brain vasculature blood vessel endothelial cell
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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