Fig. 10.
- ID
- ZDB-FIG-230420-130
- Publication
- Sigloch et al., 2023 - A network of Notch-dependent and -independent her genes controls neural stem and progenitor cells in the zebrafish thalamic proliferation zone
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Analysis of the her activity depleted herUDM mutant phenotype reveals prominent role of her6. (A-B′) Live phenotype of WT and Df(her4;12),Df(her2;15),her6,her9 mutant (herUDM) embryos, lateral views at 56 hpf; midsagittal optical section (A), parasagittal optical section at level of lens (B). (C-F″) Anti-Sox2 and anti-pH3 immunofluorescence in 72 hpf embryos, genotypes indicated. (C-F) Lateral view midline sagittal xz plane from orthogonal reconstructions. Cyan lines show dorsal view horizontal confocal planes 1 and 2 shown in C′-F′ and C″-F″, respectively (C′-F′ dorsal part of the diencephalon with the thalamus proper; C″,F″ more ventrally including the prethalamus). (C′-F′) Yellow lines show midline sagittal planes in C-F. C1-F1 show magnifications of boxed areas in C′-F′. Arrowheads in C1-F1 show Sox2low cells. Arrowheads in C″-F″ indicate the lateral expansion of ventricular Sox2high cells. Arrows show the Sox2high nuclei layer covering the ventricular wall. For confocal stacks C/D and E/F see Movies 4 and 5. A-P, anterior-posterior; D-V, dorsal-ventral; tel, telencephalon; th, thalamus; ve, ventricle. Scale bars: 50 µm (C′ for C-F″); 20 µm (C1 for C1-F1). |