FIGURE

Fig 6

ID

ZDB-FIG-230406-74

Publication

Hong et al., 2023 - Essential role of an ERV-derived Env38 protein in adaptive humoral immunity against an exogenous SVCV infection in a zebrafish model

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Fig 6

Evaluation on the functional role of Env38 in host antiviral immune defense and IgM antibody production.

(A) Survival rate was calculated in anti-Env38 Ab-mediated blockade and Env38siRNA-LV-mediated knockdown treatment in zebrafish injected with SVCV (10⁵ TCID₅₀) in continuous 21 days. Kaplan-Meier survival curves represented data pooled from three independent experiments. Statistical differences were analyzed by log-rank test. 30 zebrafish were used in each experiment (n = 30). (B) ELISA for kinetic production analysis of the anti-SVCV antibody (IgM) in serum of zebrafish with treatments of Ab-mediated blockade and siRNA-mediated knockdown of Env38 under challenge of SVCV (10⁵ TCID₅₀) after 7, 14 and 21 days. Error bars represented SEM. Isotype IgG-treated groups and scrambled siRNA-LV treated groups served as nonrelated control groups (n = 30). Negative control groups received PBS treatment. Error bars represented SEM. (*p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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