FIGURE

FIGURE 1

ID

ZDB-FIG-230406-63

Publication

Baillie et al., 2023 - The in vivo study of cardiac mechano-electric and mechano-mechanical coupling during heart development in zebrafish

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FIGURE 1

Electronic flow control system for acutely increasing cardiac preload in intact larval zebrafish. Outlet pressure of a pressurised gas cylinder is modulated by a computer-controlled high-resolution electronic pressure control system, which pressurises a conical tube filled with saline solution. Flow rate from the tube is measured by a bidirectional flow rate sensor and maintained at a set value by adjusting pressure. A micro-cannula is connected to the flow outlet, advanced with a micromanipulator under an upright fluorescence microscope to puncture the common cardinal vein (slightly upstream of where it drains into the sinus venosus), and used to inject volume into the heart. Effects of volume loading are visualised through the microscope with an industrial camera.

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.