FIGURE
Fig. 4
- ID
- ZDB-FIG-230328-27
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- Zheng et al., 2022 - A Simple, Robust, and Cost-effective Method for Genotyping Small-scale Mutations
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Fig. 4
Genotyping wild-type, heterozygous and homozygous zebrafish with 7 bp-insertion in vwf.
(a) The expected DNA sequences of the wild-type (wt) and mutant (mu) (i.e., 7 bp-insertion) in zebrafish von Willebrand factor (vwf). (b–c) are the T7E1 digestion patterns of the PCR products in the absence (−) and the presence (+) of a wt PCR product, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence and presence of a wt PCR sequence, respectively. (d–e) Sanger sequencing results confirmed the genotyping results (+7 bp-insertion) revealed by the agarose gel electrophoresis following the T7E1 digestion. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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Full text @ J Clin Transl Pathol