Figure 5

Effect of apatinib combined with pemetrexed on signaling pathways in zebrafish models. (a) Endogenous VEGFaa, VEGFR2, and VEGFR1 in control and lead compounds treated embryos were assessed by qRT-PCR (n = 10–15 individual embryos). APT, apatinib; PEM, pemetrexed; ns, not significant. (b) Endogenous Dll4, Notch1a, Hey2, and EFNB2A in control and lead compounds treated embryos were assessed by qRT-PCR (n = 10–15 individual embryos). (c) Endogenous SLIT2, SLIT3, ROBO1, ROBO2, and ROBO4 in control and lead compounds treated embryos were assessed by qRT-PCR (n = 10–15 individual embryos). (d) Endogenous FGFR11, FGFR 2, FGFR 3, and FGFR 4 in control and lead compounds treated embryos were assessed by qRT-PCR (n = 10–15 individual embryos). Zebrafish strain: Tg (fli1a:EGFP)y1. Animal number: 90 embryos for each condition.