Figure 2
Genotypes and body measurements of the prkcaa-null zebrafish. (A) Genotypes of the prkcaa−/− mutants. The target sequence was located in the seventh exon of the zebrafish prkcaa gene. Two mutant lines were generated and designated as ZKO3145A (3145A, −2 bp) and ZKO3145B (3145B, −11 bp). The target sequence, deleted nucleotides and the truncated proteins are illustrated. The red arrows indicate the joining sites of the broken DNA strands. (B–D) Measurements include body weight (B), standard length (C) and condition factor (D) for the adults of the WT and 3145A mutants. The error bars represent standard error (n = 30); ns, no significant difference. The different colored lines represent the four bases, A, T, C and G (shown above the lines). The different colored dots indicate the fish lines WT and 3145A (shown below the x-axis). |