Figure 1

Characterization of Oli-Neu cells. (A) Immunocytochemical analysis of Oli-Neu cells with MBP (red)/Olig2 (green), PLP (red)/SOX10 (green), MAG (green), and MOG (green) after 1, 2, 4, and 6 days, respectively, following stimulation with 1 µM PD 174265 compared to untreated cells. Scale bars represent 100 µm. (B) End-point PCR showed increased expression of Mag and Mog after stimulation with 1 µM PD174265. Values on the right indicate the length of the PCR product in base pairs (bp). (C) Patch-clamp experiments showed the physiological presence of voltage-gated calcium channels that could be blocked by 10 µM nimodipine and activated by 10 µM BayK8644. DMSO was used as a control. The graph displays the data points of measured electric currents (Y-axis) against the applied voltages (X-axis). Error bars indicate standard deviations. Values are representative of n = 3 independent experiments and are presented as mean ± standard deviation. Actb: beta-actin; BayK: BayK8644; bp: base pairs; Cacna1s: voltage-gated L-type calcium channel 1.1; Cacna1c: voltage-gated L-type calcium channel 1.2; Cacna1d: voltage-gated L-type calcium channel 1.3; Cacna1f: voltage-gated L-type calcium channel 1.4; DAPI: 4′,6-diamidino-2-phenylindole; DMSO: dimethyl sulfoxide; MAG: myelin-associated glycoprotein; MBP: myelin basic protein; MOG: myelin oligodendrocyte glycoprotein; ms: milliseconds; mv: millivolt; pA: picoamperes; Nimo: nimodipine; Olig2: oligodendrocyte transcription factor 2; PCR: polymerase chain reaction; PLP: proteolipid protein; SOX10: SRY-box transcription factor 10.