FIGURE

Fig. 1

ID

ZDB-FIG-230223-62

Publication

Yu et al., 2022 - Zebrafish integrin a3b is required for cardiac contractility and cardiomyocyte proliferation

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Fig. 1

Fig. 1. Itga3b is expressed in the plasma membrane of cardiomyocytes. (A) itga3b is expressed in the ventricle and atrium of embryos at 48 and 60 hpf. Images of single confocal Z-stack are shown. (B) Membrane localization of Itga3b protein is observed in both the ventricle and atrium and the epidermis near the pericardium of wild-types (a) at 72 hpf. Low level of Itga3b protein is detected in the ventricle and atrium of itga3b morphants (b). Partial membrane localization of Itga3b protein is detected in the ventricle and atrium and the epidermis near the pericardium of itga3b^+/- mutants (c). No expression of Itga3b protein is observed in the ventricle and atrium of itga3b^−/− mutants (d). (C) Itga3b protein is expressed in the heart tube at 24 and 36 hpf and in the ventricle and atrium from 48 to 72 hpf, while a low level of Itga3b protein is observed in the ventricle and atrium at 96 hpf. Dorsal view (24 hpf) and ventral views (36–96 hpf) of embryos are shown. Respective 20 (a), 13 (c) or 5 (f) Z-stacks are shown. (D) Itga3b (red) is localized to the EGFP-expressing cardiomyocye plasma membrane of Tg(myl7:EGFP-Hsa. HRAS) transgenic embryos at 72 hpf. Itga3b protein (green) is observed in the cardiomyocyte plasma membrane surrounding nuclear dsRed (red) protein in Tg(myl7:dsRed) transgenic embryos. Itga3b (red) is not found in the EGFP-expressing endocardial cells of Tg(fli1:EGFP) or in the EGFP-expressing epicardial cells of TgBAC(tcf21:NLS-EGFP) transgenic embryos. Images of single confocal Z-stack are shown. a, atrium; e, epidermis; v, ventricle. Scale bar, 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Gene:	itga3b
Antibody:	Ab1-itga3b
Fish:	WT itga3b^{sa22024/sa22024} WT + MO3-itga3b itga3b^sa22024/+ pd41Tg s879Tg s883Tg y1Tg
Knockdown Reagent:	MO3-itga3b
Anatomical Terms:	atrium atrium membrane cardiac muscle cell plasma membrane cardiac ventricle cardiac ventricle membrane endocardium epidermis membrane heart tube pericardium
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga3b^{sa22024/sa22024} WT + MO3-itga3b itga3b^sa22024/+
Knockdown Reagent:	MO3-itga3b
Observed In:	atrium membrane cardiac muscle cell plasma membrane cardiac ventricle membrane epidermis membrane
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.