FIGURE

Fig. 2.

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ZDB-FIG-230211-76

Publication

Hajiabadi et al., 2022 - Deep-learning microscopy image reconstruction with quality control reveals second-scale rearrangements in RNA polymerase II clusters

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Fig. 2.

Metric-based estimation of how far image quality can be compromised while still allowing recovery of effective resolution by denoising. (A) Representative micrographs of nuclei of human cheek cells for different camera exposure times (t_exp, as indicated), all high-quality images were acquired at the same position but with an exposure time of 200 ms. Images are maximum-intensity projections, DNA was labeled by Hoechst 33342. (B) FRC curves calculated from a pair of matched low-quality images, from a pair of reconstructed images, and a pair of high-quality images for the different t_exp. (C) Effective resolution for the indicated t_exp, n = 5 nuclei per t_exp, values are shown with mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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