FIGURE

Figure 2.

ID

ZDB-FIG-230211-113

Publication

Hamling et al., 2022 - Tilt In Place Microscopy (TIPM): a simple, low-cost solution to image neural responses to body rotations

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Figure 2.

Variability in responses arises predominantly from intrinsic sources. A, Timeline of an experiment of repeated imaging of the same fish across two mounts on the galvanometer. B, Two-photon volumes of vestibulospinal neurons in a Tg(nefma:GAL4);Tg(UAS:GCaMP6s) larva during two sequential mounting and imaging experiments. Colored overlays indicate the same neurons located in two separate volumes taken across the two mounts. C, Mean fluorescence traces (± SD) during the baseline period and following an ipsilateral roll during the first and second mount for two neurons (orange and blue traces correspond to colored neuron overlays). D, Mean peak response during trials in the first mount experiment are strongly correlated with the mean peak response from the second mount (ρ = 0.83). E, Peak response on a single experimental trial is strongly correlated with peak response on the subsequent trial within the same experiment (ρ = 0.79).

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.