FIGURE

Fig. 1

ID

ZDB-FIG-230120-9

Publication

Quadri et al., 2021 - Activation of PKG restricts melanoma growth and invasion by interfering with the EGF/EGFR pathway

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Fig. 1

Expression of PKG isoforms in melanoma spheroids. (a) RQs of the PKG-I?, PKG-I?, and PKG-II mRNAs were assessed by real-time qPCR in melanoma cell lines (WM115, WM266-4, and SKMEL-28) and human metastatic (M121224, M130425, and M000921) derived spheroids. RPS26 was used as a reference gene. RQ of WM266-4 was set as 1 for PKG-I? and PKG-I?, whereas the value of M130425 was set as 1 for PKG-II. Data represent the mean � SD of triplicate determinations. Statistical analysis by one-way ANOVA was performed comparing the mRNA level of each sample with WM115 mRNA level. (b) Immunoblotting for PKG isoforms. (c) Quantification of PKG-I?, PKG-I?, and PKG-II RQ was performed by ImageJ using ?-actin as a normalizing protein. Data represent the mean � SD of three independent biological experiments. Statistical analysis by one-way ANOVA was performed comparing the protein level of each sample with WM115 or M000921 protein levels. ??P < 0.01; ?0.01 < P < 0.05. PKG, protein kinase G; RQ, relative quantity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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