FIGURE

Fig. 5

ID

ZDB-FIG-230120-25

Publication

Gulluni et al., 2021 - PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

VPS36 recruits CHMP4B to the midbody through ALIX parallel pathway.

(A) Quantification of CHMP4B levels at midbody in HeLa cells treated with control siRNA or siRNA targeting PI3K-C2α, ALIX, or PI3K-C2α and ALIX. n ≥ 50 cells, mean ± SD. (B) Quantification of ALIX levels at midbody in HeLa cells transfected with ALIX-Cherry and treated with control or siRNA targeting PI3K-C2α or VPS36. n ≥ 60 cells, mean ± SD. (C) Quantification of CHMP4B levels at midbody in HeLa cells treated with control or siRNA targeting CHMP6. n ≥ 45 cells, mean ± SD. (D) Quantification of CHMP6 levels at midbody in HeLa cells transfected with GFP-CHMP6 and treated with control or siRNA targeting PI3K-C2α, VPS36, or ALIX. n ≥ 50 cells, mean ± SD. (E) Quantification of VPS36 levels at midbody in HeLa cells treated with control or siRNA targeting CHMP6. n ≥ 40 cells. If not previously specified, all results are shown as mean or representative picture of at least three independent experiments. (n.s. = not significant; **P < 0.01; ***P < 0.001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Science