FIGURE

Figure 3

ID

ZDB-FIG-230119-31

Publication

Richter et al., 2023 - Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio

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Figure 3

The zebrafish (Danio rerio) tenA-like thiaminase I gene encodes a functional thiaminase. Solubility and thiaminase I activity of recombinant candidate thiaminase I genes overexpressed in Escherichia coli, stained for activity showing thiamine degradation as clear areas, and with subsequent Coomassie blue protein stain. Gels are cropped to show relevant lanes and mobility range; original gels are presented in Supplementary Fig. 9. Panel A, insoluble fractions; Panel B, soluble fractions; lane 1, common carp (Cyprinus carpio), lane 2, zebrafish; lane 3, alewife (Alosa pseudoharengus); lane 4, Paenibacillus thiaminolyticus; lane 5, empty pET52b vector.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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