Fig. 3

a–d Brightfield images of 78 hpf wild-type (a), piezo1 mutant (b), piezo2a mutant (c), and piezo2a; pkd1a double mutant (d) hearts; one time point of a spinning disc movie at atrial diastole; green lines outline the heart. e AV flow profile of 78 hpf wild-type, piezo1 mutant, pkd1a mutant, piezo2a mutant, and piezo2a; pkd1a double mutant larvae; average profile of three cardiac cycles per zebrafish. f–i Confocal imaging of 78 hpf wild-type (f), piezo1 mutant (g), piezo2a mutant (h), and piezo2a; pkd1a double mutant (i) hearts stained with Bodipy FL C5-Ceramide; AV canal magnified images. j Superior AV valve elongation of 78 hpf wild types, piezo1 mutants, pkd1a mutants, piezo2a mutants, and piezo2a; pkd1a double mutants. k–n Confocal imaging of 78 hpf wild-type (k), piezo1 mutant (l), piezo2a mutant (m), and piezo2a; pkd1a double mutant (n) hearts immunostained for MHC and Fli1; AV canal magnified images; ventral views, anterior to the top, ventricle (V) on the left and atrium (A) on the right. o Superior AV valve cell number in 78 hpf wild types, piezo1 mutants, pkd1a mutants, piezo2a mutants, and piezo2a; pkd1a double mutants. Hearts imaged in ventral views, anterior to the top, ventricle (V) on the left, and atrium (A) on the right. The center of the box-and-whisker plot represents the median; each of the box-and-whiskers represents a quartile; “+” represents the mean in e. The center of the error bar represents the mean in j, o. Error bars indicate s.d in o. P values were calculated using a two-sided Student’s t-test in e, o; a two-sided Fisher’s exact test in j; and are relative to wild type except when a horizontal bar is used. Scale bars: 50 µm in a–d; 10 µm in f–i, k–n. Source data are provided as a Source Data file.