Fig. 5

Protein expression of myelin-related genes is significantly decreased in iGluSnFR-expressing 5 dpf zebrafish larvae and adult brains. (a) The expression of the myelin proteins CldnK (left) and 36K (right) is significantly decreased in iGluSnFR-expressing 5 dpf larvae (red), which is compared to iGluSnFR⁽⁻⁾ littermate controls (blue). For both proteins, the ratio of iGluSnFR⁽⁺⁾/iGluSnFR⁽⁻⁾ expression was calculated for each experiment and normalized against the average values of iGluSnFR⁽⁻⁾ expression, which was thus set to one in average, as shown. Dot plot graphs are averaged (values shown in graphs) of pixel density areas of the according Western blot bands (mean ± StDev). Data were analyzed by an unpaired two-tailed t test. p-Values are significant ***p < 0.0001; **p < 0.001; *p < 0.05; CldnK: p = 0.0001; t = 4.500; 36K: p = 0.0117; t = 2.897; N = 4 independent experiments. (b) Normalized expression of the myelin proteins CldnK (left), 36K (middle), and Mbpa (right) are significantly decreased in iGluSnFR-expressing adult brains (red) compared to controls (WT brains; blue). As above for the zfl for all protein expression quantification, the ratio of iGluSnFR⁽⁺⁾ brain/WT iGluSnFR⁽⁻⁾ expression was calculated and normalized against the average values of iGluSnFR⁽⁻⁾ expression (see methods section for details of calculations). Dot plot graphs show averaged values of pixel density areas of the according Western blot bands (mean ± StDev). Data were analyzed by an unpaired two-tailed t test. CldnK: p < 0.0001; t = 5.522; 36K: p = 0.0094; t = 2.848; Mbpa: p = 0.0007; t = 4.496. N = 3 Independent experiments. (c) Representative Western blot protein expression bands of 36K, CldnK, Mbpa, and ß-actin from 5 dpf zfl (upper panel) and adult brains (lower panel). For complete Western blot membranes, please refer to Figure S2 (zfl) and Figure S3 (adult brain)