Fig. 4

Morphology of Kupffer’s vesicle (KV) and cilia in carmil3 mutant embryos. (A) Line drawing of a 6-somite stage embryo in a lateral view with KV outlined in green. On the right side is an image of a Tg(sox17:GFP) labeled KV. (A’) Line drawing of a posterior view of the 6-somite stage embryo. This view was used to collect confocal images. On the right side is a maximum intensity z projected image showing KV. Panels B through C are from one analysis, based on wide-field fluorescence images showing the morphology of sox17-EGFP labelling of the KV in +/− carmil3^stl413 control (B) and MZcarmil3^stl413 mutant (B’ and B”') embryos at the 6-somite stage. The KV is outlined by the dashed yellow line. (C) Area of the KV in +/− carmil3^stl413 control and MZcarmil3^stl413 mutant embryos. All the data points are shown. The red lines indicate the mean and one standard error of the mean. The results are statistically significant with a p value of < 0.0001 based on Student’s t-test. The number of embryos analyzed was 15 for control and 30 for mutant. In this set of experiments, the control was a heterozygous strain. Panels D and E show results of the experiments testing if injected WT carmil3 RNA can rescue KV area in MZcarmil3 mutants. KV area was scored similarly to Figure 4 panel B. (D) Data from individual clutches are presented in the same color, e.g., orange colors are injections into a WT clutch of a control RNA, 2.5, or 5 pg of WT carmil3 RNA. Variability in ranges correlates with different clutches. Clutches used for only one injection are in grays. (E) Unpaired Student’s T-test with unequal standard deviation for rescue experiments. We plotted average area for each injection condition, with Standard Error of the Mean. Comparisons with significant p values are indicated by bars.